Genome-Wide Association Study on Three Behaviors Tested in an Open Field in Heterogeneous Stock Rats Identifies Multiple Loci Implicated in Psychiatric Disorders.

Study title

Genome-Wide Association Study on Three Behaviors Tested in an Open Field in Heterogeneous Stock Rats Identifies Multiple Loci Implicated in Psychiatric Disorders.

Description

This dataset contains results of behavioral tests as well as genotypes for 1,246 adolescent Heterogeneous Stock outbred rats. The data was collected to perform genome-wide association study.

Funding sources

P50-DA037844

Methods for genotype

Genotypes were generated as described in [PMID: 23979941, DOI: 10.1534/g3.113.007948]

Methods for Phenotypes

Open Field:Two OFT arenas were constructed using black acrylic glass, measuring 100 cm (L) × 100 cm (W) × 50 cm (H), which were placed side by side. The floors were covered by wood boards painted with either black or white acrylic paint (ART-Alternatives, ASTM D-4236, Emeryville, CA, USA) to contrast the coat of the animals (i.e., a black board was used for rats with white fur). The test chambers were illuminated by a long-range, 850-nm infrared light (LIR850-70, LDP LLC, Carlstadt, NJ) located 160 cm above the center of the two test chambers. No source of visible light was present during behavioral testing, with the exception of a flat panel monitor (Dell 1908FP). A digital camera (Panasonic WV-BP334) fitted with an 830 nm infrared filter (X-Nite830-M37, LTP LLC, Carlstadt, NJ) and located next to the infrared light source was used to record the behavior of the rats. All rats were released at the same corner of the test chamber, and data were collected for 1 hr.

Novel Object Interaction Test:This test was conducted the day after the OFT in the same arena. A cylindrical rat cage constructed using 24 aluminum rods (30 cm in length) spaced 1.7 cm apart was used as the novel object. The bottom and top of the cage (15 cm in diameter) were manufactured using a 3D printer from polylactic acid. The design can be downloaded from here. The novel object was placed in the center of the arena before testing. The test duration was 20 min and was recorded using the same camera as that used in the OFT.

Social Interaction Test:This test was conducted the day after the NOIT. This test compares the preference of a subject rat for a stimulus rat restricted in a cylindrical cage (i.e., the novel object used in the NOIT) against an empty cylindrical cage. The test arena was reduced to 100cm(L) × 60cm(W) × 50cm(H) by using a black board placed vertically in the arena. Two cylindrical cages described above were placed ~30 cm away from the walls on opposite sides (i.e., similar to the arrangement commonly used in the three-chamber test). A randomly selected stimulus Sprague-Dawley rat of the same sex and similar weight as the HS test rat was placed into one of the cylindrical cages (kept the same throughout the experiment) 5 min before the HS subject rat was placed into the arena. The stimulus and subject rats were never housed together and thus were unfamiliar to each other. No social isolation was conducted on either rat. Each stimulus rat was used no more than once per day. The test duration was 20 min and was recorded using the same camera as that used in the OFT.

Methods for Omics

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Data Freezes

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