Single-cell resolution analysis of chromatin accessibility and gene expression
We will use brains of N/NIH heterogeneous stock (HS) rats that have undergone the extended access to oxycodone self-administration procedure. This project takes advantage of a brain tissue repository of HS rats that have been genotyped and characterized as vulnerable and resistant to compulsive oxycodone use. Our preliminary studies on single-cell analysis of the cerebral cortex provide a compelling strategy to study the biological basis of opiate addiction.
Methods for genotype
low-coverage WGS based on Riptide library preparation (pulication in progress)
Methods for Phenotypes
Methods for Omics
1) Single-cell RNA-seq to identify gene expression changes in brains of HS rats; 2) Single-cell ATAC-seq to identify changes in chromatin accessibility and transcription factors binding sites; 3) H3K27Ac- PLAC-seq to link distal regulatory elements to target genes involved in oxycodone addiction-related behaviors.